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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans Progressiva
doi: 10.3390/ijms24032299
Figure Lengend Snippet: Expression of pSMAD1/5/9 in fibroblasts of FOP patients and controls by western blotting analysis. ( A ) Cells were serum-starved overnight before 60 min of stimulation with Activin A and ( B ) serum-starved overnight before 90 min of stimulation with BMP4. Actin was used to determine equal protein loading. −FBS are serum-starved unstimulated fibroblasts.
Article Snippet: Primary antibodies against phospho-SMAD3 (cat#52903, Abcam),
Techniques: Expressing, Western Blot
Journal: Nature
Article Title: Brahma safeguards canalization of cardiac mesoderm differentiation
doi: 10.1038/s41586-021-04336-y
Figure Lengend Snippet: a, Dot plots showing quantitative changes in gene expression between WT and Brm−/− cells induced with normal (1x) or high (4x) BMP4 concentrations at D4, D6 and D10 stages of differentiation for early developmental, cardiac mesoderm, precursor, and myocyte genes enriched in BRM KO cells. b-d, Transcriptional trajectory analysis of WT and BRM KO cells showing the genotype representation in normal BMP4 concentration (b), normal BMP4 for WT and 4x BMP4 concentration for BRM KO cells (c) and URD feature plots of expression of Nkx2-;5, and Actc1 (d). e, Western blots showing BMP receptor, Smad1 and phospho-SMAD expression during D0 to D4 of cardiac differentiation, f-g, Scatter plots of single cell RNASeq data showing mean gene expression and variance from mean gene expression at D4 stage of differentiation for WT (f) and Brm−/− cells (g) in low and high BMP4 conditions. h-i, Signaling entropy calculated similarly for WT (h) and Brm−/− cells (i) with low and high BMP4 conditions.
Article Snippet: Primary antibodies used were anti- BRG1 (Abcam, ab110641, 1:1000), anti-BRM (Abcam, ab240648, 1:1000), anti-FLAG (Sigma, F1804, 1:1000), anti-BAF170 (Bethyl, 1:1000, A301–39A), anti-BAF60c (Cell Signaling Technology, 62265, 1:1000), anti-REST (EMD-Millipore, 07–579, 1:1000), anti-POU3F1 (Abcam, ab126746, 1:1000), or anti-TBP (Abcam, ab51841, 1:2000), anti-Vinculin (Sigma-Aldrich V9131, 1:1000),
Techniques: Expressing, Concentration Assay, Western Blot